635nm diode laser biostimulation on cutaneous wounds

Hakan Solmaz, Murat Gülsoy, Yekta Ülgen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Citations (Scopus)

Abstract

Biostimulation is still a controversial subject in wound healing studies. The effect of laser depends of not only laser parameters applied but also the physiological state of the target tissue. The aim of this project is to investigate the biostimulation effects of 635nm laser irradiation on the healing processes of cutaneous wounds by means of morphological and histological examinations. 3-4 months old male Wistar Albino rats weighing 330 to 350 gr were used throughout this study. Low-level laser therapy was applied through local irradiation of red light on open skin excision wounds of 5mm in diameter prepared via punch biopsy. Each animal had three identical wounds on their right dorsal part, at which two of them were irradiated with continuous diode laser of 635nm in wavelength, 30mW of power output and two different energy densities of 1 J/cm2 and 3 J/cm2. The third wound was kept as control group and had no irradiation. In order to find out the biostimulation consequences during each step of wound healing, which are inflammation, proliferation and remodeling, wound tissues removed at days 3, 7, 10 and 14 following the laser irradiation are morphologically examined and than prepared for histological examination. Fragments of skin including the margin and neighboring healthy tissue were embedded in paraffin and 6 to 9 um thick sections cut are stained with hematoxylin and eosin. Histological examinations show that 635nm laser irradiation accelerated the healing process of cutaneous wounds while considering the changes of tissue morphology, inflammatory reaction, proliferation of newly formed fibroblasts and formation and deposition of collagen fibers. The data obtained gives rise to examine the effects of two distinct power densities of low-level laser irradiation and compare both with the non-treatment groups at different stages of healing process.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care IV
PublisherSPIE
ISBN (Print)9781628410778
DOIs
Publication statusPublished - 2014
Externally publishedYes
EventBiophotonics: Photonic Solutions for Better Health Care IV - Brussels, Belgium
Duration: 14 Apr 201417 Apr 2014

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9129
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care IV
Country/TerritoryBelgium
CityBrussels
Period14/04/1417/04/14

Keywords

  • Laser biostimulation
  • low-level laser therapy (LLLT)
  • therapeutic
  • wound healing

Fingerprint

Dive into the research topics of '635nm diode laser biostimulation on cutaneous wounds'. Together they form a unique fingerprint.

Cite this