Biostimulative effects of 809 nm diode laser on cutaneous skin wounds

Hakan Solmaz, Murat Gülsoy, Yekta Ülgen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

The use of low-level laser therapy (LLLT) for therapeutic purposes in medicine has become widespread recently. There are many studies in literature supporting the idea of therapeutic effects of laser irradiation on biological tissues. The aim of this study is to investigate the biostimulative effect of 809nm infrared laser irradiation on the healing process of cutaneous incisional skin wounds. 3-4 months old male Wistar Albino rats weighing 300 to 350 gr were used throughout this study. Lowlevel laser therapy was applied through local irradiation of 809nm infrared laser on open skin incisional wounds of 1 cm length. Each animal had six identical incisions on their right and left dorsal region symmetrical to each other. The wounds were separated into three groups of control, 1 J/cm2 and 3 J/cm2 of laser irradiation. Two of these six wounds were kept as control group and did not receive any laser application. Rest of the incisions was irradiated with continuous diode laser of 809nm in wavelength and 20mW power output. Two of them were subjected to laser irradiation of 1 J/cm2 and the other two were subjected to laser light with energy density of 3 J/cm2. Biostimulation effects of irradiation were studied by means of tensile strength tests and histological examinations. Wounded skin samples were morphologically examined and removed for mechanical and histological examinations at days 3, 5 and 7 following the laser applications. Three of the six fragments of skin incisions including a portion of peripheral healthy tissue from each animal were subjected to mechanical tests by means of a universal tensile test machine, whereas the other three samples were embedded in paraffin and stained with hematoxylin and eosin for histological examinations. The findings of the study show that tissue repair following laser irradiation of 809nm has been accelerated in terms of tissue morphology, strength and cellular content. These results seem to be consistent with the results of many researches previously published in literature and support the idea that LLLT has therapeutic effect on wound healing process.

Original languageEnglish
Title of host publicationMechanisms for Low-Light Therapy X
EditorsMichael R. Hamblin, Praveen Arany, James D. Carroll
PublisherSPIE
ISBN (Electronic)9781628413991
DOIs
Publication statusPublished - 2015
Externally publishedYes
EventMechanisms for Low-Light Therapy X - San Francisco, United States
Duration: 7 Feb 20158 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9309
ISSN (Print)1605-7422

Conference

ConferenceMechanisms for Low-Light Therapy X
Country/TerritoryUnited States
CitySan Francisco
Period7/02/158/02/15

Keywords

  • Laser biostimulation
  • low-level laser therapy (LLLT)
  • tensile strength
  • therapeutic
  • wound healing

Fingerprint

Dive into the research topics of 'Biostimulative effects of 809 nm diode laser on cutaneous skin wounds'. Together they form a unique fingerprint.

Cite this