TY - JOUR
T1 - Comparison of the efficacy and histopathological effects of three hemostatic agents in a partial nephrectomy rat model
AU - Yucel, Mehmet Ozgur
AU - Polat, Haci
AU - Bagcioglu, Murat
AU - Karakan, Tolga
AU - Benlioglu, Can
AU - Cift, Ali
AU - Gok, Alper
AU - Astarci, Hesna Muzeyyen
AU - Akgul, Turgay
AU - Germiyanoglu, Cankon
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Purpose: The major complications of partial nephrectomy are bleeding and urine leakage. While various hemostatic agents are used to control bleeding, the histopathological characteristics of these hemostatic agents have not been investigated adequately. We aimed to investigate and compare the histopathological and hemostatic effects of local hemostatic agents in a partial nephrectomy rat model. Methods: Thirty-two rats were divided into four equal groups, and partial nephrectomy was done to all rats. Conventional suture repair, Glubran2®, FloSeal®, and Celox™ were applied to every single group. The period of warm ischemia and hemostasis during surgical process was timed. Rats were killed later 3 weeks, and their partial nephrectomy applied kidneys were evaluated histopathologically. Results: The fastest hemostasis was provided with Glubran2® (32.87 s). FloSeal® was the second (40.85 s), and Celox™ was the third (55.75 s). Glomerular necrosis and calcification were seen more in the suture group than other groups (p < 0.001). Fibrosis was found significantly less in Celox™ group. Fibroblast activation was found significantly less comparing to other groups (p < 0.01). The erythrocyte aggregation was significantly greater in the Glubran2® and FloSeal® groups than suture group (p < 0.01 and p < 0.001). Conclusion: The negative effects of hemostatic agents to the renal histopathology were less than conventional suture repair. Celox™ was the best biocompatible agent. In comparison with three agents, it was observed that Glubran2® provided hemostasis faster than other agents.
AB - Purpose: The major complications of partial nephrectomy are bleeding and urine leakage. While various hemostatic agents are used to control bleeding, the histopathological characteristics of these hemostatic agents have not been investigated adequately. We aimed to investigate and compare the histopathological and hemostatic effects of local hemostatic agents in a partial nephrectomy rat model. Methods: Thirty-two rats were divided into four equal groups, and partial nephrectomy was done to all rats. Conventional suture repair, Glubran2®, FloSeal®, and Celox™ were applied to every single group. The period of warm ischemia and hemostasis during surgical process was timed. Rats were killed later 3 weeks, and their partial nephrectomy applied kidneys were evaluated histopathologically. Results: The fastest hemostasis was provided with Glubran2® (32.87 s). FloSeal® was the second (40.85 s), and Celox™ was the third (55.75 s). Glomerular necrosis and calcification were seen more in the suture group than other groups (p < 0.001). Fibrosis was found significantly less in Celox™ group. Fibroblast activation was found significantly less comparing to other groups (p < 0.01). The erythrocyte aggregation was significantly greater in the Glubran2® and FloSeal® groups than suture group (p < 0.01 and p < 0.001). Conclusion: The negative effects of hemostatic agents to the renal histopathology were less than conventional suture repair. Celox™ was the best biocompatible agent. In comparison with three agents, it was observed that Glubran2® provided hemostasis faster than other agents.
KW - Celox
KW - FloSeal
KW - Glubran2
KW - Hemostasis
KW - Partial nephrectomy
UR - http://www.scopus.com/inward/record.url?scp=84953637172&partnerID=8YFLogxK
U2 - 10.1007/s11255-015-1129-3
DO - 10.1007/s11255-015-1129-3
M3 - Article
C2 - 26490559
AN - SCOPUS:84953637172
SN - 0301-1623
VL - 48
SP - 65
EP - 71
JO - International Urology and Nephrology
JF - International Urology and Nephrology
IS - 1
ER -