TY - JOUR
T1 - Laser biostimulation of wound healing
T2 - bioimpedance measurements support histology
AU - Solmaz, Hakan
AU - Dervisoglu, Sergulen
AU - Gulsoy, Murat
AU - Ulgen, Yekta
N1 - Publisher Copyright:
© 2016, Springer-Verlag London.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Laser biostimulation in medicine has become widespread supporting the idea of therapeutic effects of photobiomodulation in biological tissues. The aim of this study was to investigate the biostimulation effect of laser irradiation on healing of cutaneous skin wounds, in vivo, by means of bioimpedance measurements and histological examinations. Cutaneous skin wounds on rats were subjected to 635 nm diode laser irradiations at two energy densities of 1 and 3 J/cm2 separately. Changes in the electrical properties of the wound sites were examined with multi-frequency electrical impedance measurements performed on the 3rd, 7th, 10th, and 14th days following the wounding. Tissue samples were both morphologically and histologically examined to determine the relationship between electrical properties and structure of tissues during healing. Laser irradiations of both energy densities stimulated the wound healing process. In particular, laser irradiation of lower energy density had more evidence especially for the first days of healing process. On the 7th day of healing, 3 J/cm2 laser-irradiated tissues had significantly smaller wound areas compared to non-irradiated wounds (p < 0.05). The electrical impedance results supported the idea of laser biostimulation on healing of cutaneous skin wounds. Thus, bioimpedance measurements may be considered as a non-invasive supplementary method for following the healing process of laser-irradiated tissues.
AB - Laser biostimulation in medicine has become widespread supporting the idea of therapeutic effects of photobiomodulation in biological tissues. The aim of this study was to investigate the biostimulation effect of laser irradiation on healing of cutaneous skin wounds, in vivo, by means of bioimpedance measurements and histological examinations. Cutaneous skin wounds on rats were subjected to 635 nm diode laser irradiations at two energy densities of 1 and 3 J/cm2 separately. Changes in the electrical properties of the wound sites were examined with multi-frequency electrical impedance measurements performed on the 3rd, 7th, 10th, and 14th days following the wounding. Tissue samples were both morphologically and histologically examined to determine the relationship between electrical properties and structure of tissues during healing. Laser irradiations of both energy densities stimulated the wound healing process. In particular, laser irradiation of lower energy density had more evidence especially for the first days of healing process. On the 7th day of healing, 3 J/cm2 laser-irradiated tissues had significantly smaller wound areas compared to non-irradiated wounds (p < 0.05). The electrical impedance results supported the idea of laser biostimulation on healing of cutaneous skin wounds. Thus, bioimpedance measurements may be considered as a non-invasive supplementary method for following the healing process of laser-irradiated tissues.
KW - Bioimpedance measurement
KW - Biostimulation
KW - Photobiomodulation (PBM)
KW - Therapeutic
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84976466631&partnerID=8YFLogxK
U2 - 10.1007/s10103-016-2013-9
DO - 10.1007/s10103-016-2013-9
M3 - Article
C2 - 27371448
AN - SCOPUS:84976466631
SN - 0268-8921
VL - 31
SP - 1547
EP - 1554
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 8
ER -