TY - JOUR
T1 - Polyenylphosphatidylcholine pretreatment protects rat liver from ischemia/reperfusion injury
AU - Demirbilek, Savaş
AU - Karaman, Abdurrahman
AU - Gürünlüoǧlu, Kubilay
AU - Taş, Erkan
AU - Akin, Melih
AU - Aksoy, Rauf Tuǧrul
AU - Türkmen, Emine
AU - Edali, Mehmet Naci
AU - Baykarabulut, Aysun
PY - 2006/2
Y1 - 2006/2
N2 - Background: Hepatic injury induced by ischemia/reperfusion following surgery, transplantation, or circulatory shock combined with resuscitation is a major clinical problem. Polyenylphosphatidylcholine (PPC) has strong antioxidant, cytoprotective and anti-inflammatory effects. Aim: In this study, the influence of PPC pretreatment on ischemia-reperfusion (I/R) injury of the liver was examined in rats. Methods: The animals were divided into three groups: control (n = 10), I/R (n = 15) and I/R + PPC (n = 15). PPC was given 100 mg/day for 7 days before experiment. Several parameters of hepatic damage, oxidative stress, neutrophil infiltration and nuclear factor kappa beta (NF-κB) expression were measured as well as microscopic examination. Results: We observed that a significant reduction in AST and ALT values in the PPC treated group when compared with the ischemic group. The increases in hepatic total NO2 + NO3 and MDA, and decreases in SOD and GSH levels after reperfusion were partially, but significantly, inhibited by PPC pretreatment. I/R induced increase in hepatic myeloperoxidase content and NF-κB expression were also lowered by PPC pretreatment. Animals pretreated with PPC presented minimal hemorrhage and reduced signs of liver injury. Conclusion: PPC pretretament provided significant protection againts I/R injury to the liver. This treatment could be therapeutic in liver transplantation and other conditions associated with I/R injury.
AB - Background: Hepatic injury induced by ischemia/reperfusion following surgery, transplantation, or circulatory shock combined with resuscitation is a major clinical problem. Polyenylphosphatidylcholine (PPC) has strong antioxidant, cytoprotective and anti-inflammatory effects. Aim: In this study, the influence of PPC pretreatment on ischemia-reperfusion (I/R) injury of the liver was examined in rats. Methods: The animals were divided into three groups: control (n = 10), I/R (n = 15) and I/R + PPC (n = 15). PPC was given 100 mg/day for 7 days before experiment. Several parameters of hepatic damage, oxidative stress, neutrophil infiltration and nuclear factor kappa beta (NF-κB) expression were measured as well as microscopic examination. Results: We observed that a significant reduction in AST and ALT values in the PPC treated group when compared with the ischemic group. The increases in hepatic total NO2 + NO3 and MDA, and decreases in SOD and GSH levels after reperfusion were partially, but significantly, inhibited by PPC pretreatment. I/R induced increase in hepatic myeloperoxidase content and NF-κB expression were also lowered by PPC pretreatment. Animals pretreated with PPC presented minimal hemorrhage and reduced signs of liver injury. Conclusion: PPC pretretament provided significant protection againts I/R injury to the liver. This treatment could be therapeutic in liver transplantation and other conditions associated with I/R injury.
KW - Hepatic ischemia/reperfusion
KW - Lecithin
KW - Liver ischemia/reperfusion
KW - Oxidative stress
KW - Polyenylphosphatidylcholine
UR - http://www.scopus.com/inward/record.url?scp=31344448765&partnerID=8YFLogxK
U2 - 10.1016/j.hepres.2005.09.040
DO - 10.1016/j.hepres.2005.09.040
M3 - Article
AN - SCOPUS:31344448765
SN - 1386-6346
VL - 34
SP - 84
EP - 91
JO - Hepatology Research
JF - Hepatology Research
IS - 2
ER -
