Özet
Objective: The aim of this study was to determine the rate of Her-2 gene amplification in breast cancer cases with a previous negative Her-2 result as determined by immunohistochemistry (score 0 or 1). Material and Method: 552 cases of invasive breast carcinoma were assessed with the contribution of 9 centers. Previous immunohistochemistry score was either 0 or 1+ in all cases. These cases were re-tested by Her-2 silver in situ hybridization in the central laboratory. Her-2 gene amplification was defined as Her-2/CEP 17 ratio of more than 2.2. Cases with a ratio between 1.8 and 2.0 were defined as equivocal and cases with a ratio of less than 1.8 were defined as negative. Results: Re-testing of the 552 cases with silver in situ hybridization showed a total of 22 cases with Her-2 gene amplification, of which 11 (3.2%) were found to be score 0, and 11 were found to be score 1+ (5.3%) by immunohistochemistry previously. Her-2 gene amplification rate of cases (score 0 and 1+) ranged from 0% to 10.48% among the centers. Polysomy was found in 28 (8.1%) of the score 0 cases and 25 (12.1%) among the score 1+ cases. Five (9.4%) of the cases with polysomy were found to be amplified, and 48 (90.6%) were not. Conclusion: The results of the study show that a group of cases (3.98%) with a potential to benefit from anti-Her-2 therapy may be missed with the immunohistochemical method. This indicates the importance of quality assurance, especially in central laboratories with many breast cancer cases in daily practice.
Tercüme edilen katkı başlığı | Her-2 durumu i̇mmünohistokimya ile negatif olan i̇nvaziv meme karsinomlari{dotless}nda i̇n situ hibridizasyon yöntemi ile deǧerlendirme (Ulusal çok merkezli çali{dotless}şma) |
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Orijinal dil | İngilizce |
Sayfa (başlangıç-bitiş) | 87-93 |
Sayfa sayısı | 7 |
Dergi | Turk Patoloji Dergisi/Turkish Journal of Pathology |
Hacim | 30 |
Basın numarası | 2 |
DOI'lar | |
Yayın durumu | Yayınlanan - 2014 |